怎么查看网站啥系统做的,免费软件看电视剧,网站建设与管理好处,去哪网网站设计风格Enzo Life Sciences的GFP-CERTIFIED FLUOFORTE Calcium assay kit提供了一种荧光分析方法#xff0c;用于检测广泛生物靶标的细胞内钙动员情况。相对于其他商业化的染料#xff0c;GFP-CERTIFIED FLUOFORTE染料是最亮和最灵敏的荧光钙指示剂。该试剂盒为贴壁和非贴壁细胞系提…Enzo Life Sciences的GFP-CERTIFIED® FLUOFORTE® Calcium assay kit提供了一种荧光分析方法用于检测广泛生物靶标的细胞内钙动员情况。相对于其他商业化的染料GFP-CERTIFIED® FLUOFORTE®染料是最亮和最灵敏的荧光钙指示剂。该试剂盒为贴壁和非贴壁细胞系提供了更少步骤更低变异性更简易的钙检测。此外它既不需要洗涤步骤也不需要添加外源性淬灭染料避免了对受体-配体相互作用动力学的干扰。
Enzo Life Sciences/欣博盛生物的GFP-CERTIFIED® FLUOFORTE® Calcium assay kit可用于细胞内钙离子的高通量检测钙离子是重要的第二信使常用于初步筛选中监测G蛋白偶联受体激活。 产品特色
● 可监测表达GFP细胞中的钙离子动员情况
● 最明亮、最灵敏的荧光Ca2指示剂
● 获得的 EC50和IC50值与使用钙离子荧光探针 Rhod-4 AM和Fluo-4 AM 相当
● 更大范围的检测窗口
● 使用方便无需洗涤无需淬灭染料 实验示例 图2. U-2 OS细胞分别与AGFP-CERTIFIED® FLUOFORTE®AM染料和BRhod-2 AM染料孵育。洗涤细胞并用终浓度为100nM的ATP处理并立即成像。GFP-CERTIFIED® FLUOFORTE® AM染料比Rhod-2 AM表现出更亮的荧光强度。 产品信息 产品货号 ENZ-51020-KP010/ ENZ-51020-KP100 产品名称 GFP-CERTIFIED® FLUOFORTE® Calcium assay kit / 欣博盛生物 规 格 10*96wells/100*96wells 短期保存 -20°C 长期保存 -80°C 试剂盒组分 Starter pack (Prod. No. ENZ-51020-KP010): Reagent A (lyophilized GFP-CERTIFIED® FLUOFORTE® dye), 1 vial Reagent B (dye efflux inhibitor), 10ml Reagent C (Hanks’ buffer with 20mM HEPES, 100ml) Sufficient for 10 x 96-well plates High-throughput (Prod. No. ENZ-51020-KP100): Reagent A (lyophilized GFP-CERTIFIED® FLUOFORTE® dye), 10 vials Reagent B (dye efflux inhibitor), 10 x 10ml Sufficient for 100 x 96-well plates 部分产品引用文献
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